In vitro fertilization is  a method used  for  recovery of mice line from cryopreserved sperm . It may also be used  as a method to overcome male fertility problems.

In vitro fertilization can be used to produce large colonies of mice in a relatively short period of time, saving also on mouse caging space.  However, the success of IVF relies largely on that reasonable number of motile sperm can be retrieved. Therefore considering this method one should be awared, that reproductive differences between mouse strains seen in vivo are also evident in vitro. Males used for cryopreservation and IVF should be at age between 3-5 months  and should  have already proven fertility before.