Viral transgenesis method is based on the delivery of transgenes into embryos via recombinant viruses. For this purpose only viruses able to integrate into the host genome are used. This feature constitutes the basis for persistent infection and transmission of the incorporated transgene to the progeny. HIV-derived lentiviral (LV) vectors retained integrating capabilities of parental virus. Lentiviral transgenesis is a well established alternative to pronuclear microinjection technique. In this method viral particles are injected into the perivitelline space of fertilized zygote (subzonal injection), what is far less invasive than standard microinjection, as the embryo membranes remain intact. Using lentivectors as a tool for transgene delivery can result in an increased survival rate of the zygotes close to 100% and even 90% founder animals. 

Pronuclear injection (see DNA microinjection) method often results in the random integration of concatamers of incorporated transgene. On the other hand, lentiviral transduction leads to multiple integrations, each containing a single copy of transgene. This can result in ‘losing’ copies of transgene in every next generation, as integrants will probably segregate independently. Therefore, it may be  optimal to establish stable lines carrying single insertions. Since viral titer seems to be correlated with the number of insert copies, the latter can be experimentally modified according to your researcher needs.

  Sample submission

 

 

The concentration of viral suspension must be checked before the experiment starts (Real-Time PCR). You can order quantitative analysis of your sample at LAM.

All information regarding animal strains, performance guarantee, turnaround times, documents submission, etc., can be found in DNA microinjection section.