Sperm Cryopreservation techniques enables you to conserve resources when you are not actively using a model and to ensure your valuable strains are protected from unfortunate events, including natural disasters, facility failures, disease outbreaks, breeding cessation and genetic drift. By allowing our reproductive science experts to cryopreserve your research strain, you leverage our unmatched capabilities for successful cryorecovery. Sperm Cryopreservation  allows you to:

           Cryopreserve your line quickly and inexpensively - only 3-4 males needed

            Achieve reliable recovery of archived sperm 

            Rapidly build a colony of specified and opportunistic pathogen free (SPF) mice

            Distribute your strain to collaborators easily

Sperm cryopreservation has three significant biological disadvantages compared to embryo cryopreservation:

           Since a haploid genome is being frozen, more breeding of reconstituted mice may be necessary if more than one mutation and/or transgene is involved, or if homozygosity is needed.

           Some mutations must be maintained on a mixed or otherwise unusual background to maintain the desired phenotype, and oocyte donors with that background may not be available at the time of reconstitution.

           As mitochondria are inherited exclusively from the maternal lineage, cryopreservation of sperm cannot be used to preserve strains of mice with mtDNA polymorphisms.

 Also, despite recent improvements in the efficiency of reconstitution from frozen sperm, a very small percentage of strains are still extremely difficult to revive from frozen sperm. Thus, in some cases, embryo cryopreservation may still be necessary.

This service is avaiable for mouse only. To order mouse sperm cryopreservation please fill in the form and contact us.

Sperm_Cryopreservation_Form.pdf